The present work had as objective to evidence the influence of the parasite Habronema muscae on the Musca domestica longevity. Muscids larvae belonging to second generation, with aproximately 30 h old, were submitted to infection in artificial diet, in which were inoculated H. muscae eggs, obtained from maceration of female helminths. The muscid larvae and pupae were mantained in artificial chamber at 27º C, 60 ± 10% RH and 14 h of photophase. After emergence, the adults were distributed in screen cages, The number of dead muscids were conferred daily, with posterior dissection and ratification of infective nematode larvae. The mean intensity of infection was 3.11 infective larvae per muscid (range: 0-14) in the infected group. The infected group showed a significative reduction on mean longevity, when compared with the control (25.0 and 32.5 days, respectively). There was no difference between mean longevity from male and female dipterous.

QUALITY CONTROL OF Cochliomyia macellaria (FABRICIUS) (DIPTERA: CALLIPHORIDAE) IMATURES IN COLONY Quality control of Cochliomyia macellaria (Fabricius) (Diptera: Calliphoridae) larvae and pupae in colony was observed during eight consecutive generations. A diet consisting of decomposing horse flesh was used, and the relation of 0.02 g egg mass/ 200 g diet was established. The larval and pupal viability at 27 oC and 30 oC temperature, 65 <FONT FACE=Symbol>±</FONT> 10% UR and 14 h photofase, respectively, were greater than 80%. The egg to pupae period and the body mass of postfeeding larvae variation were 4.7-7.0 days and 59.40-70.30 mg, respectively. The emergence ocurred 8.0-12.0 days after the neolarvae innoculation in diet. The study has not show a linear relation between the analised characteristic and monitored generations

RESUMO O controle de qualidade de larvas e pupas de Cochliomyia macellaria (Fabricius) (Diptera: Calliphoridae), mantidas em estoques, foi monitorado durante oito gerações consecutivas. Uma dieta consistindo de carne eqüina putrefata foi utilizada, estabelecendo-se a relação de 0.02 g de massa de ovos / 200 g de dieta. A taxa de sobrevivência larval e pupal a 27 ºC e 30 ºC de temperatura, 65 <FONT FACE=Symbol>±</FONT> 10% UR e 14 h de fotofase, respectivamente, foi superior a 80%. O período de ovo a pupa e a massa corporal de larvas pós-alimentadas oscilou entre 4.7-7.0 dias e 59.40-70.30 mg, respectivamente. A emergência ocorreu 8.0-12.0 dias após a inoculação das neolarvas na dieta. O estudo não sugeriu uma relação linear entre as características analisadas e as gerações monitoradas.

In the present study we estimed the longevity and reproductive potential of Cochliomyia macellaria (Fabricius, 1775), in different densities of grouped pairs, as a function of time of collection. The study was divided into two phases and conducted in an incubator regulated at 27oC, 65±10% RH and 14 hours photoperiod. Forty and 20 pairs/replication were used, corresponding to the third and fourth generation originating from a laboratory colony. Egg masses were collected at 8:00 and 17:00 hours daily. The results obtained were influenced by the variables established. The ovipositing peak occurred 20 days after the emergence of females belonging to the third generation. This peak occurred earlier in fourth generation females, i.e., 11 days after imago emergence. These peaks corresponded to a total of 0,30 and 0,23g of egg masses, respectively. The survival curves for adults were estimated by the Weibull method.

This paper has the purpose of studying the interspecif relationships of larvae of Cochliomyia macellaria (Fabricius) and Chrysomya albiceps (Wiedemann) in laboratory. The experiments were conducted on climatized chamber set at 30ºC, 60+10% UR and 14h photophase. The relation of 1 larva/g diet was established. The association influence of post-embrionary development duration of both species. The association with Chrysomya albiceps influenced the weight of mature larvae of Cochliomyiia macellaria. reducing it significantly. Such weight decrease, along with the predation of larvae of C. macellaria larvae, yielded a decrease in survival in all of the stages.

This paper intends to recognize some aspects of interspecific relationships between Chrysomya megacephala (Fabricius) and C. albiceps (Wiedemann) and between C. megacephala and Cochliomyia macellaria (Fabricius) larvae in laboratory. A diet consisting of decomposing horse flesh was used, and the relation of 1 larva/g diet was established. The development of the two species was done in pure and associated cultures. The association with Chrysomya albiceps influenced the post-embrionary development period and the weight of mature larvae of the C. megacephala, reducing their. Such weight decrease, along with the predation of larvae of C. megacephala larvae, yelded a decrease in survival in all of the stages. Larvae of this species showed an increase in its weight when associated with Cochliomyia macellaria. The native species responded to the association by reducing its body weigth and its viability. This association doesnt influence the duration of post-embrionary development.

The possible dissimilitudes relating to the duration of the egg incubation period and larval stage of Cochliomyia macellaria (Fab., 1775) from offsprings of females from larvae with and without pigmentation on Malpighi tubules, morphotype 1 and 2, respectively, was questioned. The experiment was conducted in climatized chamber regulated at 30ºC, 65±10% RH and 14 hour photophase. No significant difference was observed on average duration of first and second larval instars berween the two morphotypes, taking into account not only the conventional interpretation for the reading of different development phases of the larval stages (morphotype 1:11.0 and 11.9 hours and morphotype 2:11.7 and 12.7 hours for first and second instars, respectively), but also the reading made through the interpretation preconized by Snodgrass & Hinton (morphotype 1:10.3 and 9.6 hours and morphotype 2:9.8 and 10.8 hours for the first and second instars, respectively). The incubation period was significantly longer for eggs posited by females from non-pigmented larvae.

The colony of C. albiceps (Wiedemann, 1819) was established from larvae and adults colected in the "Estação para Pesquisa Parasitológica W.O. Neitz" area at UFRRJ and Seropedica District, Itaguai, Rio de Janeiro. (Latitude: 22º45'S; Longitude: 43º41'W and Altitude: 33 metres). The biological aspects of this species were studied in climatized chambers regulated at 60±10% RH and 14 hours photophase. The duration and the viability of the larval and pupal stages of C. albiceps was 5,2 and 4,53 days; 95,75% and 94%, respectively. An effect of group was observed on the larvae at the first and second instar. The average weight of mature larvae was around 75 mg, varying between 12,2 to 120 mg. Only mature larvae with average weight higher than 42 mg have originated adults. Oviposition peaks between the sixth and tenth days pos-emergence were observed. Survival curves of the males and females were estimated using the Weibull model.